Question

Write steps involved in gene amplification.

Answer 1

Denaturation: The reaction mixture is heated to a temperature (90–98 oC) to separate two strands of desired DNA. This is called denaturation

Annealing: The mixture is allowed to cool (40–60 oC) that permits pairing of the primer to the complementary sequences in DNA. This step is called annealing

Extension: The temperature (70–75 oC) allows thermostable Taq DNA polymerase to use single-stranded DNA as template and adds nucleotides. This is called primer extension. It takes arround two minutes duration.