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Question
"A very small sample of tissue or even a drop of blood can help determine paternity." Provide a scientific explanation to substantiate how it is possible.
Solution
A very small sample of DNA obtained from a tissue or even a drop of blood can serve as a source for paternity testing. By using the techniques of PCR and DNA fingerprinting, we can determine the paternity of any individual.
The steps are as follows -
- The first step includes the extraction of DNA from the cells or blood.
- The DNA molecules are then broken into small fragments that contain a variable number of tandem repeats (VNTR's).
- The DNA fragments are then separated by gel electrophoresis.
- These DNA fragments are multiplied using PCR.
- They are then converted into single-stranded DNA by treatment with alkaline chemicals.
- It is followed by transferring these fragments to the nylon membrane.
- The nylon membrane is exposed to X-ray film which creates dark-colored bands called fingerprints.
- These fingerprints can then be used to determine paternity.
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RELATED QUESTIONS
The genetic marker used as key factor in DNA fingerprinting is _______.
(A) VNTRs
(B) Exons
(C) Introns
(D) DNA probes
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VNTR has ______.
DNA having variable length obtained by restriction digestion is called ______.
The process in which sample DNA and probe DNA form double stranded DNA is called ____________.
DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as ______
Which of the following is not true with respect to INA fingerprinting?
- DNA fingerprinting technique was first developed by Alec Jeffrey.
- DNA profiling is a more recent and more sensitive version of DNA fingerprinting.
- DNA fingerprinting was first used in Britain to verify the parentage of an immigrant, who had left the country and then wanted to return
- In DNA fingerprinting, single stranded radioactive DNA is used as probes.
Choose the correct option.
