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Question
Define vector? write any two examples,
Solution
- Cloning vectors are DNA molecules that carry a foreign DNA segment and replicate inside the host cell.
- Following are the different types of vectors used in rDNA technology: Vectors may be plasmids, bacteriophages (M13, lambda virus).
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| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Write steps involved in gene amplification.
Write a short note on vectors.
What is EcoRI?
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
