Question

Give the basic steps in r-DNA technology

Answer 1

Steps involved in rDNA technology:

•  Identifying the donor cell and isolating its DNA
• Fragmenting the DNA using ‘molecular scissors’ (restriction enzymes)
•  Screening the fragmented DNA for the desired gene
•  Inserting the desired DNA fragment into a cloning vector to produce the recombinant DNA
•  Introducing the recombinant vector organism in the desired host cell
•  Culturing the recombinant cells to obtain multiple copies of the desired DNA fragment
• These DNA fragments are then used to transform desired host cells where the expression of the gene is desired Therapeutic products obtained by rDNA technology