Advertisements
Advertisements
Question
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
Solution
- The Bt toxin is encoded by the cry gene. The cry I AC and Cry II Ab control cotton boll worms, while the cry I Ab controls corn borer. Bt gene produces Bt toxin. This toxin provides resistance to plants against lepidopteron, coleopteron and dipterans pests.
- An example is Bt cotton, in which the gene from the bacterium that encodes for the toxin is incorporated. The activated Bt toxin binds to the surface of the midgut epithelium of the insects and causes swelling and cell lysis, which eventually leads to the death of the insects.
- In the process of producing insect-resistant crops, specific Bt toxin genes are isolated from B.thuringiensis, and incorporated into the crops. Since these toxins are insect specific, they do not harm the crops or humans.
APPEARS IN
RELATED QUESTIONS
Alu-I is obtained from which organism?
Match the List I with List II and List ill. Select the correct option.
List I | List II | List ill | |||
i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
iii. | T-DNA | c. | Ampicillin | 3. | Elution |
iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
The vector Baculovirus is used for gene transfer in ____________.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as ______.
Which of the following is not required in the preparation of a recombinant DNA molecule?
Describe three steps involved in mechanism of PCR.
Write steps involved in gene amplification.
Which amino acid is coded by the initiation codon?