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Question
Name the components of lac operon and discuss their role.
Solution
The various components of lac operon are regulator gene, promoter gene, operator gene and structural genes. Regulatory genes synthesize a chemical called repressor which combine with operator gene to switch off the operon. In the presence of inducer – the lactose, repressor binds with lactose to free the operator gene and the operon is switch on. The promoter gene is the recognition site for RNA polymerase when the operon is switched on. The RNA polymerase pass over the operator gene and reach structural genes to transcribe them. Operator gene controls the expression of operon by switching on and off the structural genes transcribe polycistronic in-RNA. Lac operon has three structural genes Z, Y, and A. The polycistronic m-RNA produces three enzymes galactosidase permease (early of lactose into bacterium), β-galactosidase (breakage of lactose into glucose and galactosidase) and galactoside acetylase (transfer acetyl group to β-galactosidase).
Note: Inducer is a chemical generally a substrate binds with a repressor to free the operator's gene.
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RELATED QUESTIONS
Short Answer Question:
Explain the role of lactose in ‘Lac Operon’.
In lac operon, lactose acts as:
Which of the following is the function of promoter in lactose operon of Escherichia coli?
Identify the INCORRECT statement/s.
- Regulation of lac operon by repressor is referred to as negative regulation.
- The repressor protein produced by regulator gene binds to the operator region.
- The sequence constituting the operator region is present upstream of promoter region
- Each operon bas a specific 'Operator'.
Which of the following is TRUE with reference to Operon?
In which of the following conditions the lac operon in E. coli becomes "switched on"?
Lactose operon produces enzymes ______.
In lac operon, lactose is the substrate for enzyme beta-galactocidase and its regulates ______.
Which of the following is required as an inducer(s) for the expression of lac operon?
Select the correct pair.