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प्रश्न
Name the components of lac operon and discuss their role.
उत्तर
The various components of lac operon are regulator gene, promoter gene, operator gene and structural genes. Regulatory genes synthesize a chemical called repressor which combine with operator gene to switch off the operon. In the presence of inducer – the lactose, repressor binds with lactose to free the operator gene and the operon is switch on. The promoter gene is the recognition site for RNA polymerase when the operon is switched on. The RNA polymerase pass over the operator gene and reach structural genes to transcribe them. Operator gene controls the expression of operon by switching on and off the structural genes transcribe polycistronic in-RNA. Lac operon has three structural genes Z, Y, and A. The polycistronic m-RNA produces three enzymes galactosidase permease (early of lactose into bacterium), β-galactosidase (breakage of lactose into glucose and galactosidase) and galactoside acetylase (transfer acetyl group to β-galactosidase).
Note: Inducer is a chemical generally a substrate binds with a repressor to free the operator's gene.
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संबंधित प्रश्न
Describe how the lac operon operates, both in the presence and absence of an inducer in E.coli.
A unit of lac-operon which in the absence of lactose, suppresses the activity of operator gene is
Enlist the genes in Lac operon
What is meant by an operon?
Explain the role of Lactose as an inducer in Lac-operon.
Which of the following is NOT the part of an operon?
From the following which is NOT the component of operon?
- Regulator gene
- Promoter gene
- Inducer
- Structural genes
- Operator gene
E. coli cells with a mutated Z gene of the lac operon cannot grow in medium containing only lactose as the source of energy because ______.
Select the correct pair.
The diagram given below represents the image of the cloning vector pUC 18. The gene of interest is inserted and ligated within the gene lacZ. The recombinant DNA is introduced in the host bacterial cell. Explain the method that would help in selection of recombinant colonies from non-recombinant colonies.