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प्रश्न
The diagram given below represents the image of the cloning vector pUC 18. The gene of interest is inserted and ligated within the gene lacZ. The recombinant DNA is introduced in the host bacterial cell. Explain the method that would help in selection of recombinant colonies from non-recombinant colonies.
उत्तर
Blue-white selection
LacZ codes for β-galactosidase
The product (β-galactosidase) becomes inactive due to insertion of foreign protein (coded by foreign gene).
Chromogenic substrate converted into blue coloured product in non-recombinant colonies, remains colourless in recombinant colonies.
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संबंधित प्रश्न
Give one significant contribution to the following scientists:
G. Gamow
Short Answer Question:
Describe the structure of ‘Operon’.
In lac operon, lactose acts as:
A unit of lac-operon which in the absence of lactose, suppresses the activity of operator gene is
What is meant by an operon?
In E.coli, the lac operon gets switched on when ______.
In lac operon, lactose is the substrate for enzyme beta-galactocidase and its regulates ______.
Which molecule does act as inducer molecule in lac operon?
Which gene acts as a regulatory gene in lac operon?
Which one of the following is not a part of lac operon?
