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Question
The diagram given below represents the image of the cloning vector pUC 18. The gene of interest is inserted and ligated within the gene lacZ. The recombinant DNA is introduced in the host bacterial cell. Explain the method that would help in selection of recombinant colonies from non-recombinant colonies.
Solution
Blue-white selection
LacZ codes for β-galactosidase
The product (β-galactosidase) becomes inactive due to insertion of foreign protein (coded by foreign gene).
Chromogenic substrate converted into blue coloured product in non-recombinant colonies, remains colourless in recombinant colonies.
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RELATED QUESTIONS
Multiple Choice Question:
Which out of the following is not an example of an inducible operon?
Enlist the genes in Lac operon
Which of the following is the function of promoter in lactose operon of Escherichia coli?
In lac operon concept of gene expression, allolactose acts as, ____________.
In Escherichia coli lac Operon is induced by ______.
The wild type E. coli cells are growing in normal medium with glucose. They are transferred to a medium containing only lactose as sugar. Which of the following changes takes place?
During expression of an operon, RNA polymerase binds to ______.
Assertion (A): Regulation of lac operon by a repressor is referred to as negative regulation.
Reason (R): Lac operon is under the control of positive regulation as well.
Which of the following is required as an inducer(s) for the expression of lac operon?
Which molecule does act as inducer molecule in lac operon?
