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प्रश्न
The diagram given below represents the image of the cloning vector pUC 18. The gene of interest is inserted and ligated within the gene lacZ. The recombinant DNA is introduced in the host bacterial cell. Explain the method that would help in selection of recombinant colonies from non-recombinant colonies.
उत्तर
Blue-white selection
LacZ codes for β-galactosidase
The product (β-galactosidase) becomes inactive due to insertion of foreign protein (coded by foreign gene).
Chromogenic substrate converted into blue coloured product in non-recombinant colonies, remains colourless in recombinant colonies.
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संबंधित प्रश्न
Name the components of lac operon and discuss their role.
Short Answer Question:
Explain the role of lactose in ‘Lac Operon’.
What is meant by an operon?
With respect to lac- operon explain the following terms:-
- regulator gene
- promoter gene
- structural gene
- inducer
Which of the following is TRUE with reference to Operon?
Lactose operon produces enzymes ______.
The wild type E. coli cells are growing in normal medium with glucose. They are transferred to a medium containing only lactose as sugar. Which of the following changes takes place?
Assertion (A): Regulation of lac operon by a repressor is referred to as negative regulation.
Reason (R): Lac operon is under the control of positive regulation as well.
Glucose is a ______.
Which enzyme will produce in a cell in which there is a nonsense mutation in the lac Y-gene?
