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Question
Name the regions marked A, B and C.
Solution
A - Bam HI
B - Pst I
C - Ampicillin resistance gene (ampR)
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RELATED QUESTIONS
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Why is the coding sequence of an enzyme β - galactosidase a preferred selectable marker in comparison to the ones named above?
The genetic material of virus includes:
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
Which of the following is correctly matched?
Which of the following statement is not true for a clone?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
