Advertisements
Advertisements
Question
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
Options
Denaturation of template DNA
Annealing of primers to template DNA
Extension of primer end on the template DNA
Extension of primer end on the template DNA
Solution
Extension of primer end on the template DNA
APPEARS IN
RELATED QUESTIONS
Explain briefly:
PCR
Answer the following question:
Describe the process of amplification of the "gene of interest" using the PCR technique.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?
In PCR, primers are used for ______.
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
How is copy number of the plasmid vector related to yield of recombinant protein?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
