Advertisements
Advertisements
प्रश्न
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
विकल्प
Denaturation of template DNA
Annealing of primers to template DNA
Extension of primer end on the template DNA
Extension of primer end on the template DNA
उत्तर
Extension of primer end on the template DNA
APPEARS IN
संबंधित प्रश्न
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Answer the following question:
Describe the process of amplification of the "gene of interest" using the PCR technique.
Which of the following term used for defining a viral genome incorporated into host DNA?
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
How is copy number of the plasmid vector related to yield of recombinant protein?
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
State the advantage of using Thermostable DNA polymerase.
