हिंदी

Which of the following contributed in popularising the PCR (polymerase chain reactions) technique? - Biology

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प्रश्न

Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?

विकल्प

  • Easy availability of DNA template

  • Availability of synthetic primers

  • Availability of cheap deoxyribonucleotides

  • Availability of 'Thermostable' DNA polymerase

MCQ

उत्तर

Availability of 'Thermostable' DNA polymerase

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Processes of Recombinant DNA Technology
  क्या इस प्रश्न या उत्तर में कोई त्रुटि है?
अध्याय 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७६]

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एनसीईआरटी एक्झांप्लर Biology [English] Class 12
अध्याय 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 10. | पृष्ठ ७६

संबंधित प्रश्न

Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.


Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.


Answer the following question:
Describe the process of amplification of the "gene of interest" using the PCR technique.


______ plays an important role in blood clotting.


In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?


An antibiotic resistance gene in a vector usually helps in the selection of ______.


While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?


Read the paragraph given below and answer and questions that follow:

Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique.
  1. Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
  2. What is a primer and its importance in PCR?
  3. Write the importance of PCR as a diagnostic tool.

Read the following paragraph and answer the questions that follow:

Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy.
  1. Name the human disease for which the gene therapy was used for the first time.
  2. Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
  3. Write the possible permanent cure of the disease by the gene therapy that is in progress. 

Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.

Reason (R): The primers bind to the double stranded DNA at their complementary regions.


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