Advertisements
Advertisements
Question
Why are bio-fertilizers preferred over chemical fertilizers?
Solution
Biofertilizers are preferred for chemical fertilizers due to the following advantages :
(i) Decomposition products of biofertilizers help to bring mineral constituents of soil into solution.
(ii They improve physiochemical properties of soil such as cation exchange and buffering action.
(iii) They modify the physical properties like granulation of the soil and increases permeability and moisture-holding capacity of the soil.
(iv) They are economically cheaper than chemical fertilizers.
(v) Biofertilizers do not cause any harm to the environment while many chemical fertilizers are non-degradable and cause soil and water pollution.
(vi) They provide food for soil microbes and thus enhance microbial activities.
APPEARS IN
RELATED QUESTIONS
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
Give one significant difference between:
Electroporation and Gene Gun.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Which of the following is an INCORRECT match?
Which of the following DNA sequence is recognised and cut by EcoR I?
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Describe three steps involved in mechanism of PCR.
Write a short note on vectors.
