Advertisements
Advertisements
Question
Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.
Solution
Recombinant DNA technology is used to obtain foreign gene product.
It involves following steps-
1. Identification and isolation of gene of interest
2. Adding or ligating the gene of interest into suitable vector, resulting in formation of a complete recombinant DNA molecule
3. Transfer of this recombinant DNA molecule into suitable host cell
4. Selection of recombinants and non-recombinant cells
5. Expression of gene of interest by culturing recombinant cells under suitable conditions
6. Culturing of cells in bioreactors for large scale production of desired product
APPEARS IN
RELATED QUESTIONS
Why must a cell be made ‘competent’ in biotechnology experiments? How does calcium ion help in doing so?
Mention the type of host cells suitable for the gene guns to introduce an alien DNA.
State the role of “biolistic gun” in biotechnology experiments.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Plasmids
Why should a bacterium be made ‘competent’ ?
Explain the role of ‘microinjection’ and ‘gene gun’ in biotechnology
For transformation, micro particles coated with DNA to be bombarded with gene gun are made up of ______.
Non viral and non vector methods are sometimes used to transfer genes or alien DNA into a plant cell. Explain one such method used in genetic engineering.
