Advertisements
Advertisements
प्रश्न
Explain how recombinant human insulin was prepared in 1983 by Eli Lily an American company.
उत्तर
1983, Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin.
APPEARS IN
संबंधित प्रश्न
What is gene therapy? Name the first clinical case in which it was used.
C-peptide of human insulin is ______.
Choose the correct option regarding retrovirus.
Which of the following is the correct sequence of steps in a PCR (Polymerase Chain Reaction)?
Now a days it is possible to detect the mutated gene causing cancer by allowing a radioactive probe to hybridize its complimentary DNA in a clone of cells, followed by its detection using autoradiography because ______
With regard to insulin choose the correct options.
- C-peptide is not present in mature insulin.
- The insulin produced by rDNA technology has C-peptide.
- The pro-insulin has C-peptide.
- A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.
For effective treatment of the disease, early diagnosis and understanding of its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
List the disadvantages of insulin obtained from the pancreas of slaughtered cows and pigs:
List the advantages of recombinant insulin.
