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प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
उत्तर
The correct sequence of the steps involved in biotechnological reaction are
Genomic DNA template
↓
Denaturation of ds-DNA
↓
Nucleotides provided
↓
Primers
↓
Thermostable DNA polymerase (from Thermus aquaticus )
↓
Enzyme DNA polymerase
↓
Chemically synthesised oligonucleotides
↓
Complementary region of DNA
↓
In vitro synthesis of copies of DNA of interest
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संबंधित प्रश्न
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Expand the following abbreviation: BAC
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Which of the following DNA sequence is recognised and cut by EcoR I?
____________ cuts the DNA within the specific positions.
Annealing step of PCR operates at ______ °C.
Study the following lists and choose the correct match.
| List I | List II |
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| B. Downstream processing | (ii) Mobile genetic elements |
| C. Cry II AB | (iii) Controls corn borers |
| D. Transposons | (iv) Ti plasmid |
| (v) Purifying protein in biopharmaceuticals |
Write steps involved in gene amplification.
Name the source of thermostable DNA polymerase.
