Advertisements
Advertisements
प्रश्न
What would be the molar concentration of human DNA in a human cell?
उत्तर
Molar concentration is the number of moles of solute in a solution divided by its volume, given in litres.
The average weight of a DNA base pair = 650 daltons (1 dalton equals the mass of a single hydrogen atom or 1.67 × 10-24 grams)
The molecular weight of a double-stranded DNA molecule = Total no. of base pairs × 650 daltons.
The human genome is 3.3 × 109 base pairs in length.
Hence, the weight of the human genome
= 3.3 × 109 base pair × 650 Daltons
= 3.59 × 10-12 gm.
The molar concentration of DNA can be calculated accordingly.
APPEARS IN
संबंधित प्रश्न
The biological scissor is ______.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
In vitro amplification of DNA or RNA segment is known as ___________.
Explain the properties of a good or ideal cloning vector for rDNA technology.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Ti plasmid being used for introducing genes in plants obtained from ______.
What is plasmid?
What is Palindromic sequence?
Bt-cotton shows adverse effect on the population of which butterfly?
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
The time taken to complete one cycle of PCR is around ______.
Which of the following is an INCORRECT match?
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
Which of the following viruses has the capacity to produce lysozymes?
Who among the following was awarded the Nobel Prize for the development of PCR technique?
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
Plasmid is ______.
