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प्रश्न
Compare TLC and Paper Chromatography techniques.
उत्तर
| Chromatography technique | TLC | Paper chromatography |
| Principle | It is based on the principle of differential adsorption. Different solutes are adsorbed on an adsorbent to a different extent. | It is based on the continuous differential partitioning of components of a mixture between stationary and mobile phases. |
| Stationary phase | Solid (adsorbent like silica gel or alumina over a glass plate) | Liquid (water trapped in the fibres of a paper) |
| Mobile phase | Liquid (single solvent/mixture of solvents) | Liquid (single solvent/mixture of solvents) |
| Visualization of components of a mixture | Similar to TLC the coloured components are visible as coloured spots and the colourless components are observed under UV light or using a spraying agent. | |
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संबंधित प्रश्न
Explain the principle of paper chromatography.
The best and latest technique for isolation, purification and separation of organic compounds is ______.
What is chromatography?
Name the simplest type of chromatography?
On what principle is this method based?
What is meant by stationary phase and mobile phase in chromatography?
Name the different types of chromatographic techniques.
Give a scientific reason.
In chromatography, the ingredients of a mixture rise up to limited height water rises up to the upper end of the paper.
State the correct technique for the separation of the following mixture.
solid constituents in a liquid constituent by adsorption.
Dyes of an ink
- Give the principle involved in the separation of the mixture
- Give the technique of separation of the mixture.
Give names of two materials used as stationary phase in chromatography.
How do you visualize colourless compounds after separation in TLC and Paper Chromatography?
Label the diagram and explain the process in your words.
Chromatography is based on the principle of ______.
During hearing of a court case, the judge suspected that some changes in the documents had been carried out. He asked the forensic department to check the ink used at two different places. According to you which technique can give the best results?
What is paper chromatography?
The separation of two coloured substances was done by paper chromatography. The distances travelled by solvent front, substance A and substance B from the baseline are 3.25 cm, 2.08 cm and 1.05 cm respectively. The ratio of Rf values of A to B is ______.
In chromatography, which of the following statement is INCORRECT for Rf?
