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How do you visualize colourless compounds after separation in TLC and Paper Chromatography? - Chemistry

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प्रश्न

How do you visualize colourless compounds after separation in TLC and Paper Chromatography?

टीपा लिहा

उत्तर

  1. Thin-layer chromatography (TLC): If components are colourless but have the property of fluorescence then they can be visualized under UV light, or the plate can be kept in a chamber containing a few iodine crystals. The iodine vapours are adsorbed by the components and the spots appear brown. Also, a spraying agent like ninhydrin can also be used (for amino acids).
  2. Paper Chromatography: The spots of the separated colourless components may be observed either under ultra-violet light or by the use of an appropriate spraying agent.
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पाठ 3: Basic Analytical Techniques - Exercises [पृष्ठ ३४]

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बालभारती Chemistry [English] 11 Standard
पाठ 3 Basic Analytical Techniques
Exercises | Q 2. (P) | पृष्ठ ३४

संबंधित प्रश्‍न

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