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प्रश्न
Describe the role of high temperature.
उत्तर
The first step of PCR is denaturation. In this step, high temperature is required for denaturation of the DNA strands i.e. separating the double-stranded DNA into single strands. Since the two strands are held together by hydrogen bonding, a high temperature is required to break down these bonds and separate the two strands.
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