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प्रश्न
How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
उत्तर
Conventionally, Insulin was isolated and refined from the pancreas of pigs and cows to treat diabetic patients. Though it is effective, due to minor structural changes, the animal insulin caused allergic reaction in a few patients.
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संबंधित प्रश्न
Explain enzyme – replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
Suggest any two possible treatments that can be given to a patient exhibiting adenosine deaminase deficiency.
State the role of C peptide in human insulin.
Can you suggest a method to remove oil (hydrocarbon) from seeds based on your understanding of rDNA technology and chemistry of oil?
Explain how insulin can be produced using recombinant DNA technology.
Why does Bt toxin not kill the bacterium that produces it, but kill the insect that ingests it?
Give a schematic representation of the transformation of a pro-insulin into insulin.
Answer the following question.
Explain the various steps involved in the production of artificial insulin.
The first clinical gene therapy was done for the treatment of ________.
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Vaccines that use components of a pathogenic organism rather than the whole organism are called ____________.
How is the amplification of a gene sample of interest carried out using PCR?
What are DNA vaccines?
PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
What are recombinant vaccines? Explain the types.
Explain how recombinant Insulin can be produced.
A patient suffering from diabetes mellitus will have ______.
A probe which is a molecule used to locale-specific sequences in a mixture of DNA or RNA Mole cubs could be ______.
The site of production of ADA the body is ______.
ELISA is based on:
When gene targetting involving gene amplification is attempted in an individual’s tissue to treat disease, it is known as ______
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
Now a days it is possible to detect the mutated gene causing cancer by allowing a radioactive probe to hybridize its complimentary DNA in a clone of cells, followed by its detection using autoradiography because ______
With regard to insulin choose the correct options.
- C-peptide is not present in mature insulin.
- The insulin produced by rDNA technology has C-peptide.
- The pro-insulin has C-peptide.
- A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.
Why is it that the line of treatment for a genetic disease is different from infectious diseases?
Taking examples under each category, discuss upstream and downstream processing.
List the advantages of recombinant insulin.
A host cell must be made competent, before it is able to receive an rDNA. Justify.
