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प्रश्न
How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
उत्तर
Conventionally, Insulin was isolated and refined from the pancreas of pigs and cows to treat diabetic patients. Though it is effective, due to minor structural changes, the animal insulin caused allergic reaction in a few patients.
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संबंधित प्रश्न
Write the function of adenosine deaminase enzyme.
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Explain how insulin can be produced using recombinant DNA technology.
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With regard to insulin choose the correct options.
- C-peptide is not present in mature insulin.
- The insulin produced by rDNA technology has C-peptide.
- The pro-insulin has C-peptide.
- A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.
For effective treatment of the disease, early diagnosis and understanding of its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
The Adenosine deaminase deficiency results in ______.
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Eli Lilly's contribution for diabetic patients through r-DNA technology has been overwhelmingly accepted. Explain how?
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
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The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
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