Advertisements
Advertisements
प्रश्न
Discuss briefly how a probe is used in molecular diagnostics.
उत्तर
A single-stranded DNA or RNA, tagged with a radioactive molecule (probe) is allowed to hybridise to its complementary DNA in a clone of cells followed by detection using autoradiography. The clone having the mutated gene will hence not appear on the photographic film, because the probe will not have complementarity with the mutated gene.
APPEARS IN
संबंधित प्रश्न
Explain enzyme – replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
What is gene therapy? Name the first clinical case in which it was used.
Describe the role of high temperature.
How many amino acids are arranged in the two chains of Insulin?
How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
Explain how ADA deficiency can be corrected?
For effective treatment of the disease, early diagnosis and understanding of its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
How is a mature, functional insulin hormone different from its prohormone form?
Explain how recombinant human insulin was prepared in 1983 by Eli Lily an American company.
The graph given below indicates the administration of the first (L) and second dose (M) of a vaccine. The corresponding response of the body is indicated by X, and Y. Interpret the graph and explain the reason for such a response shown by the body.
