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प्रश्न
Explain how ADA deficiency can be corrected?
उत्तर
The right approach for SCID treatment would be to give the patient a functioning ADA which breaks down toxic biological products.
In some children ADA deficiency could be cured by bone marrow transplantation, where defective immune cells could be replaced with healthy immune cells from a donor. In some patients it can be treated by enzyme replacement therapy, in which functional ADA is injected into the patient.
During gene therapy the lymphocytes from the blood of the patient are removed and grown in a nutrient culture medium. A healthy and functional human gene, ADA cDNA encoding this enzyme is introduced into the lymphocytes using a retrovirus. The genetically engineered lymphocytes are subsequently returned to the patient. Since these cells are not immortal, the patient requires periodic infusion of such genetically engineered lymphocytes. The disease could be cured permanently if the gene for ADA isolated from bone marrow cells are introduced into the cells of the early embryonic stages.
संबंधित प्रश्न
State the cause of ADA deficiency in humans.Mention a possible cure for an ADA deficiency patient.
Write any two biochemical/molecular diagonostic procedures for early detection of viral infection. Explain the principle of any one of them.
The genetic defect adenosine deaminase deficiency may be cured permanently by ____________.
ELISA is mainly used for ____________.
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
What is genetically engineered Insulin?
Explain how “Rosie” is different from a normal cow.
What are recombinant vaccines? Explain the types.
A patient suffering from diabetes mellitus will have ______.
Human insulin is being commercially produced forma transgenic species of ______.
Choose the correct option regarding retrovirus.
Which of the following is not an application of PCR (Polymerase Chain Reaction)?
For effective treatment of the disease, early diagnosis and understanding of its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
Can a disease be detected before its symptoms appear? Explain the principle involved.
What is a recombinant DNA vaccine? Give two examples.
How is a mature, functional insulin hormone different from its prohormone form?
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
How many cycles of PCR are required to produce 250 molecules of DNA, starting with a single parental strand?
