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प्रश्न
Which one of the following statements is true regarding DNA polymerase used in PCR?
पर्याय
It is used to ligate introduced DNA in recipient cells
It serves as a selectable marker
It is isolated from a Virus
It remains active at a high temperature
उत्तर
It remains active at a high temperature
संबंधित प्रश्न
Explain how insulin can be produced using recombinant DNA technology.
Why does Bt toxin not kill the bacterium that produces it, but kill the insect that ingests it?
Write any two biochemical/molecular diagonostic procedures for early detection of viral infection. Explain the principle of any one of them.
Describe the steps that are followed during secondary treatment of sewage.
The first clinical gene therapy was done for the treatment of ________.
ELISA technique is based on the principles of antigen-antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?
Which of the following is a neuropeptide hormone?
C-peptide of human insulin is ______.
Due to which of the following reasons insulin extracted from pancreas of slaughtered animals cannot be used in humans to treat diabetes?
When gene targetting involving gene amplification is attempted in an individual’s tissue to treat disease, it is known as ______
Now a days it is possible to detect the mutated gene causing cancer by allowing a radioactive probe to hybridize its complimentary DNA in a clone of cells, followed by its detection using autoradiography because ______
Discuss briefly how a probe is used in molecular diagnostics.
Who was the first patient who was given gene therapy? Why was the given treatment recurrent in nature?
When a foreign DNA is introduced into an organism, how is it maintained in the host and how is it transferred to the progeny of the organism?
Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
A cell-free method of amplifying DNA first developed in the mid 1980's revolutionised the field of biotechnology, Name the method and explain the basic steps of the technique involved.
Explain how recombinant human insulin was prepared in 1983 by Eli Lily an American company.
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
How many cycles of PCR are required to produce 250 molecules of DNA, starting with a single parental strand?
