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प्रश्न
During in vitro synthesis of DNA, a researcher used 2’, 3’ – dideoxy cytidine triphosphate as raw nucleotide in place of 2’-deoxy cytidine. What would be the consequence?
उत्तर
Further polymerisation would not occur, as the 3’ OH on sugar is not there to add a new nucleotide for forming ester bond.
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संबंधित प्रश्न
If a double stranded DNA has 20 per cent of cytosine, calculate the per cent of adenine in the DNA.
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A short piece of DNA, having 20 base pairs, was analyzed to find the number of nucleotide bases in each of the polynucleotide strands. Some of the results are shown in the table.
Number of nucleotide bases | ||||
Adenine | Cytosine | Guanine | Thymine | |
Strand 1 | 4 | 4 | ||
Strand 2 | 5 |
How many nucleotides containing Adenine were present in strand 2?