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प्रश्न
Give the applications of DNA fingerprinting technique.
Mention DNA fingerprinting application.
List any two applications of DNA fingerprinting technique.
Mention any two applications of DNA fingerprinting technique.
उत्तर
- In parental disputes, the DNA fingerprinting technique is used to determine the true parents of the child.
- In forensic science, DNA fingerprinting is used to determine the culprit of the crime by matching the DNA fingerprint of the suspect with the biological evidence (hair, blood, skin cells, etc.) found at the crime scene.
- It is a sure-shot tool to solve paternity or maternity suits because the VNTR pattern of an individual is inherited from both the parents involved.
Notes
Students should refer to the answer according to their questions and provided marks.
APPEARS IN
संबंधित प्रश्न
Briefly describe the following:
Polymorphism
Which material is used for isolation of DNA in fingerprinting technique?
Name the radioactive probe used in India for DNA fingerprinting.
State the best-known contribution of Alec Jeffery
Short Answer Question:
Match the entries in column I with those of column II and choose the correct answer.
| Column I | Column II |
| A. Alkali treatment | i. Separation of DNA fragments on gel slab |
| B. Southern blotting | ii. Split DNA fragments into single strands |
| C. Electrophoresis | iii. DNA transferred to nitrocellulose sheet |
| D. PCR | iv. X-ray photography |
| E. Autoradiography | v. Produce fragments of different sizes |
| F. DNA treated with REN | vi. DNA amplification |
Long Answer Question:
Guess (i) the possible location of DNA on the collected evidence from a crime scene and (ii) the possible sources of DNA.
| Evidence | Possible location of DNA on the evidence | Sources of DNA |
| e.g. Eyeglasses | e.g. Ear pieces | e.g. Sweat, Skin |
| Bottle, Can, Glass | Sides, mouthpiece | __________ |
| __________ | Handle | Sweat, skin, blood |
| Used cigarette | Cigarette butt | ____________ |
| Bite mark | ____________ | Saliva |
| __________ | Surface area | Hair, semen, sweet, urine |
Define 'RFLP’.
Explain different steps involved in DNA Fingerprinting.
Restriction endonucleases cut the DNA molecule at specific positions known as ______.
In gene cloning the DNA of interest is integrated in a ______.
State the applications of DNA fingerprinting technique.
In DNA fingerprinting technique, which of the following probe is used for hybridization of DNA fragments?
Which of the following technique is used for amplification or multiplication of DNA for fingerprinting?
Identify the CORRECT description of DNA fingerprinting.
____________ are the DNA sequences which are useful in DNA fingerprinting.
ln which of the following technique DNA bands are transferred from agarose gel to nitrocellulose or nylon membrane?
Which of the following is the technique developed to identify a person with the help of DNA restriction analysis?
The process in which sample DNA and probe DNA form double stranded DNA is called ____________.
Match the Column - I with Column - II and select the correct option
| Column - I | Column - II | ||
| (a) | Female banded Krait | (i) | Humulin |
| (b) | Pancreas from dog | (ii) | DNA sample |
| (c) | Hair root of human | (iii) | Insulin extracted and purified |
| (d) | E.coli | (iv) | DNA probe |
Select the mis-matched pair.
Short stretches of DNA used to identify the complementary sequences in a sample are called ______
Which one of the following is an incorrect statement with regard to pedigree analysis?
The DNA fingerprinting pattern of the child is ______
Which of the following technique is used m DNA fingerprinting?
Would it be appropriate to use DNA probes such as VNTR in DNA finger printing of a bacteriaphage?
DNA fragments formed by the action of restriction enzyme in a chemical reaction can be separated by ______.
The technique of DNA fingerprinting was first given by ______.
Enlist the steps in DNA fingerprinting giving its applications.
