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प्रश्न
Name the radioactive probe used in India for DNA fingerprinting.
उत्तर
In India, the unique segment obtained from the Y chromosome of the female banded krait snake i.e. Banded Krait minisatellite is used as a radioactive probe for DNA fingerprinting.
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संबंधित प्रश्न
“A very small sample of tissue or even a drop of blood can help determine paternity”. Provide a scientific explanation to substantiate the statement.
Following the collision of two trains a large number of passengers are killed. A majority of them are beyond recognition. Authorities want to hand over the dead to their relatives. Name a modern scientific method and write the procedure that would help in the identification of kinship.
In DNA fingerprinting technique, radioactive DNA probe is obtained from______ of female banded krait snake.
(a) X chromosome
(b) Y chromoson1e
(c) X and Y chromosomes
(d) autosome
How is repetitive/satellite DNA separated from bulk genomic DNA for various genetic experiments?
"A very small sample of tissue or even a drop of blood can help determine paternity." Provide a scientific explanation to substantiate how it is possible.
Short Answer Question:
Match the entries in column I with those of column II and choose the correct answer.
Column I | Column II |
A. Alkali treatment | i. Separation of DNA fragments on gel slab |
B. Southern blotting | ii. Split DNA fragments into single strands |
C. Electrophoresis | iii. DNA transferred to nitrocellulose sheet |
D. PCR | iv. X-ray photography |
E. Autoradiography | v. Produce fragments of different sizes |
F. DNA treated with REN | vi. DNA amplification |
Which of the following technique is used for amplification or multiplication of DNA for fingerprinting?
Identify the enzyme used for cutting DNA segment in genetic engineering.
Select the mis-matched pair.
Arrange the following steps of DNA fingerprinting in correct sequence:
- Gel electrophoresis
- Isolation of DNA
- Southern blotting
- Restriction digestion