Question

Answer the following question.

Explain ETS.

Answer 1

1. NADH₂ and FADH₂ produced during glycolysis, connecting link reaction and Krebs cycle are oxidized with the help of various electron carriers and enzymes.
2. These carriers and enzymes are arranged on the inner mitochondrial membrane in the form of various complexes as complex I, II, III, VI and V.
3. NADH+H⁺ is oxidized by NADH dehydrogenase (complex I) and it's electrons are transferred to ubiquinone (coenzyme Q-CoQ) present on the inner membrane of mitochondria. Reduced ubiquinone is called as ubiqunol.
4. FADH₂ is oxidized by complex II (Succinate dehydrogenase) and these electrons are also transferred to CoQ.
5. During oxidation of NADH+H⁺ and FADH₂, electrons and protons are released but only electrons are carried forward whereas protons are released into the outer chamber of mitochondria (intermembrane space).
6. Ubiquinol is oxidized by complex-III (Cytochrome bc1 complex) and it's electrons are transferred to cytochrome C. Cytochrome C is a small, iron-containing protein, loosely associated with the inner membrane. It acts as a mobile electron carrier, transferring the electrons between complex III and IV.
7. Cytochrome C is oxidized by complex IV or cytochrome C oxidase consisting of cytochrome a and a₃. Electrons are transferred by this complex to the molecular oxygen. This is terminal oxidation.
8. Reduced molecular oxygen reacts with protons to form a water molecule called as metabolic water.
9. Protons necessary for this are channelled from the outer chamber of mitochondria into the inner chamber by F₀ part of oxysome (complex V) present in the inner mitochondrial membrane. This proton channelling by F₀ is coupled to the catalytic site of F₁ which catalyses the synthesis of ATP from ADP and inorganic phosphate. This is oxidative phosphorylation. As the transfer of protons is accompanied with a synthesis of ATP, this process is named as 'Chemiosmosis' by Peter Mitchell.