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Question
Can you recall meiosis and indicate at what stage a recombinant DNA is made?
Solution
In meiosis, the number of chromosomes is reduced to half. In the first meiotic division, one or more segments are exchanged between homologous chromosomes of each pair, i.e., they cross over.
Homologous chromosomes form pairs in the zygotene substage of the first prophase of meiotic division. This process is called synapsis. In the pachytene substage, round microscopic knobs appear at one or more places in the synaptonemal complex, these are called recombination nodules.
The mutual exchange of one or more segments between the interconnected chromatids of homologous chromosomes is called crossing over. This results in the formation of homologous recombinant DNA. Recombination nodes are formed at the sites where pieces of chromatids break and rejoin for transduction.
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