Advertisements
Advertisements
Question
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
Solution
Thermus aquaticus is the source organism for the thermostable DNA polymerase used in PCR, and its polymerase is popularly known as Taq polymerase.
RELATED QUESTIONS
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Describe briefly the following:
Downstream processing
Explain briefly:
Chitinase
Answer the following question:
Describe the process of amplification of the "gene of interest" using the PCR technique.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?