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प्रश्न
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
उत्तर
Thermus aquaticus is the source organism for the thermostable DNA polymerase used in PCR, and its polymerase is popularly known as Taq polymerase.
संबंधित प्रश्न
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Explain briefly:
Chitinase
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
The first step in recombinant DNA technology is
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
