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Question
cDNA means ______.
Options
coiled DNA
cytoplasmic DNA
complementary DNA
circular DNA
Solution
cDNA means complementary DNA.
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RELATED QUESTIONS
In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.
Explain the steps in process of rDNA technology with suitable diagrams.
Explain the following terms with respect to rDNA technology
- passanger DNA
- Chimeric DNA
- Transformed cell
- restriction site
Study the following statements regarding proteins that are being produced by transgenic crop plants and select the correct option.
i. Human testosterone hormone is produced by gene inserted into the chloroplast DNA of tobacco plants.
ii. Protein antigens to be used in vaccines for e.g. Patient-specific antilymphoma (a cancer) vaccines.
iii. In transgenic plants, Humanized antibodies can be produced against HIV, Respiratory syncytial virus (RSV), Herpes simplex virus (HSY).
Which of the following is used for assisting the transfer of rDNA into a bacterial cell?
Which of the following technique is used for the separation of DNA fragments of different lengths?
Identify the techniques that can be used to transfer foreign DNA directly into the cell or protoplast without the use of plasmids.
i. Biolistic method
ii. Ultrasonification
iii. Shot gun
iv. Lipofection
v. Microinjection
vi. Electroporation
Identify the process by which competent host can take up chimeric DNA.
Identify the process in Column I with the term in Column II and select the correct option.
| Column I | Column II |
| A. Amplification of gene of interest | I. Downstream processing |
| B. Large-scale production of plants in a short duration. | II. Polymerase chain reaction |
| C. Separation and purification of the desired product | III. Transformation |
| D. Insertion of rDNA into the host | IV. Micropropagation |
Give reason: All the fragments of a genome are cloned for storing them in genomic library.
