Advertisements
Advertisements
Question
Which molecules are called molecular scissors? Why are they called so?
Solution
Restriction enzymes are called molecular scissors.
Each restriction endonuclease inspects the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar-phosphate backbones. Each restriction endonuclease recognises specific palindromic nucleotide sequences in the DNA.
APPEARS IN
RELATED QUESTIONS
Describe two vectors less methods of gene transfer used in rDNA technology.
Enlist and write in brief about the different biological tools required in rDNA technology.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
From the following identify the methods of electrophoresis.
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
Annealing step of PCR operates at ______ °C.
The PCR technique is used for ______.
Which of these are most widely used in genetic engineering?
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.