Question

Write four sequential steps of the procedure of the experiment "Preparing a temporary mount of a leaf peel to show stomata".

Answer 1

The steps involved in the preparation of temporary leaf mount to view stomata  are:

1. Remove a part of the peel from the lower surface of the leaf and place the peel in a watch glass containing water.

2. Put a few drops of safranin stain in the watch glass and after 2-3 minutes, approximately take out the peel and place it on a clean glass slide.

3. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle to avoid any kind of air bubbles.

4. Remove the excess stain and glycerin with the help of blotting paper. and observe the slide under the microscope.