Advertisements
Advertisements
प्रश्न
ELISA technique is based on the principles of antigen-antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?
उत्तर
Yes. One can use antibody against the enzyme (that is responsible for the metabolism of phenylalanine) to develop ELISA based diagnostic technique. The patient where the enzyme protein is absent would give negative result in ELISA when compared to the normal individuals.
APPEARS IN
संबंधित प्रश्न
Why do children cured by enzyme-replacement therapy adenosine deaminase deficiency need periodic treatment?
Write the function of adenosine deaminase enzyme.
Can you suggest a method to remove oil (hydrocarbon) from seeds based on your understanding of rDNA technology and chemistry of oil?
Consult internet and find out how to make orally active protein pharmaceutical. What is the major problem to be encountered?
Why does Bt toxin not kill the bacterium that produces it, but kill the insect that ingests it?
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
The first clinical gene therapy was done for the treatment of ________.
The genetic defect adenosine deaminase deficiency may be cured permanently by ____________.
PCR proceeds in three distinct steps governed by temperature, they are in order of ____________.
Which one of the following statements is true regarding DNA polymerase used in PCR?
ELISA is mainly used for ____________.
How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
What are DNA vaccines?
What are recombinant vaccines? Explain the types.
Which of the following is a neuropeptide hormone?
Human insulin is being commercially produced forma transgenic species of ______.
Due to which of the following reasons insulin extracted from pancreas of slaughtered animals cannot be used in humans to treat diabetes?
Which of the following is the correct sequence of steps in a PCR (Polymerase Chain Reaction)?
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
Who was the first patient who was given gene therapy? Why was the given treatment recurrent in nature?
Define Antigen and Antibody. Name any two diagnostic kits based upon them.
A host cell must be made competent, before it is able to receive an rDNA. Justify.
Explain how recombinant human insulin was prepared in 1983 by Eli Lily an American company.
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
