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प्रश्न
Explain how recombinant Insulin can be produced.
उत्तर
Production of insulin by recombinant DNA technology started in the late 1970s. This technique involved the insertion of human insulin gene on the plasmids of E. coli. The polypeptide chains are synthesized as a precursor called pre-pro insulin, which contains A and B segments linked by a third chain C and preceded by a leader sequence. The leader sequence is removed after translation and the C chain is excised, leaving the A and B polypeptide chains Explain the steps involved in the production of recombinant hGH.
Using recombinant DNA technology hGH can be produced. The gene for hGH is isolated from the human pituitary gland cells. The isolated gene is inserted into a plasmid vector and then is transferred into E. coli. The recombinant E. coli then starts producing human growth hormone. The recombinant E. coli are isolated from the culture and mass production of hGH is carried out by fermentation technology.
संबंधित प्रश्न
State the role of C peptide in human insulin.
Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
Expand the Given and mention one application of each:
ELISA
State the best-known contribution of P.K. Sethi
Describe the steps that are followed during secondary treatment of sewage.
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
Describe the role of primers.
How many amino acids are arranged in the two chains of Insulin?
Which one of the following statements is true regarding DNA polymerase used in PCR?
ELISA is mainly used for ____________.
What is genetically engineered Insulin?
ELISA technique is based on the principles of antigen-antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?
Human insulin is being commercially produced forma transgenic species of ______.
C-peptide of human insulin is ______.
The Adenosine deaminase deficiency results in ______.
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
Eli Lilly's contribution for diabetic patients through r-DNA technology has been overwhelmingly accepted. Explain how?
The graph given below indicates the administration of the first (L) and second dose (M) of a vaccine. The corresponding response of the body is indicated by X, and Y. Interpret the graph and explain the reason for such a response shown by the body.
Illustrate using any one example of transgenic bacteria.
