Advertisements
Advertisements
प्रश्न
Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
उत्तर
1. For each cycle of PCR two primers are required.
2. Primers are the small fragments of single stranded DNA or RNA that serve as a template for initiating DNA polymerization.
3. DNA polymerase is an enzyme that synthesizes DNA molecules by pairing the Deoxyribo Nucleotides leading to the formation of new strands.
4. DNA polymerase used in PCR is Taq polymerase which is isolated from a thermophilic bacteria called Thermus aquatics.
5. Taq polymerase will remain active ever at a very high temperature (80°C) and hence used in the PCR amplification technique.
संबंधित प्रश्न
Explain enzyme – replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
How did an American Company, Eli Lilly use the knowledge of r-DNA technology to produce human insulin?
Expand the Given and mention one application of each:
PCR
State the role of C peptide in human insulin.
Can you suggest a method to remove oil (hydrocarbon) from seeds based on your understanding of rDNA technology and chemistry of oil?
What is gene therapy? Name the first clinical case in which it was used.
Write any two biochemical/molecular diagonostic procedures for early detection of viral infection. Explain the principle of any one of them.
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
The genetic defect adenosine deaminase deficiency may be cured permanently by ____________.
Which one of the following statements is true regarding DNA polymerase used in PCR?
ELISA is mainly used for ____________.
A patient suffering from diabetes mellitus will have ______.
A pathophysiology is the ______.
Due to which of the following reasons insulin extracted from pancreas of slaughtered animals cannot be used in humans to treat diabetes?
Which is false about antibiotics?
When a foreign DNA is introduced into an organism, how is it maintained in the host and how is it transferred to the progeny of the organism?
Eli Lilly's contribution for diabetic patients through r-DNA technology has been overwhelmingly accepted. Explain how?
- Assertion: PCR is a powerful technique to identify genetic disorders.
- Reason: PCR can detect mutations in low amounts of DNA.
