Advertisements
Advertisements
Question
Describe the role of CaCl2 in the preparation of competent cells?
Solution
CaCl2 is known to increase the efficiency of DNA uptake to produce transformed bactrial cells. The divalent Ca+2 ions supposedly create transient pores on the bactrial cell wall by which the entry of foreign DNA is facilitated into the bactrial cells.
APPEARS IN
RELATED QUESTIONS
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
A clone is a ______.
Which of the following is correctly matched?
A suitable vector for gene cloning in higher organism is ______.
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Name the regions marked A, B and C.
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
