Advertisements
Advertisements
Question
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
Solution
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids is a metabolic burden to the microbial cells, will thus tend to lose the plasmid.
APPEARS IN
RELATED QUESTIONS
What does PCR stand for? Describe the different steps of PCR
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
Enlist and write in brief about the different biological tools required in rDNA technology.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
EcoRl & Hind III are the examples of ______
The main reason for the presence of both a leading and a lagging strand during DNA replication is, ______
What is meant by gene cloning?
