Give a reason for the following: The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide. - Biology (Theory)

Give a reason for the following:

The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.

One Line Answer

More than one restriction site for the same RE.

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Q 1.xviii.a | 1 mark

Give an account of the Blue-White Method of selection of recombinants

Describe two vectors less methods of gene transfer used in rDNA technology.

In vitro amplification of DNA or RNA segment is known as ___________.

The recognition sequence of restriction enzymes is generally ______ nucleotide long.

Identify the CORRECT sequence of steps in PCR.

Study the following statements regarding separation of DNA using agarose gel electrophoresis.

Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
DNA being negatively charged migrates to cathode.

Name two restriction enzymes used in PCR.

______technique is used for in vitro gene cloning or gene multiplication.

Explain the mechanism of PCR.

Study the diagram given below and answer the questions that follow.

Name the cloning vector shown above. In which organism in this cloning vector inserted?
Mention any two restriction sites shown in the diagram.
Name any two selectable markers shown in the diagram.